The Stem Cell Unit offers comprehensive support in the context of the generation, cultivation, characterisation, genome editing and differentiation of patient-specific iPS cells. All services offered are carried out according to established and validated SOPs and include the planning and execution of projects, as well as a primary evaluation of results. The general conditions are specified in our terms of use. Detailed service options and respective charges can be found in our catalogue of services and can be discussed upon request. We recommend to make contact at an early stage for a better co-ordination of the planning of experiments and the preparation of samples as well as to ensure the prompt processing of requests.
The laboratory service of the Stem Cell Unit comprises the following:
(1) Reprogramming in iPS cells
(a) Establishing of patient-specific primary cell cultures
(b) Reprogramming of patient-specific fibroblasts or mononucleated blood cells into iPS cells (non-integrating method, feeder- and serum-free culture conditions)
(c) Characterization of generated iPS cell lines including the analysis of pluripotency and differentiation potential, as well as analysis of genome stability
(2) Genome editing of iPS cells according to latest technologies
gene knockout, gene knockin and SNP insertion approaches by CRISPR/Cas9
(3) iPS cell differentiation in various cell types
currently primarily in functional cardiomyocytes
(4) Stem cell biobank
Provision of patient-specific iPS cell lines and derivatives thereof
For detailed information please refer to the current cost and service catalogue (german).